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Data availability
The density maps and structure coordinates have been deposited to the Electron Microscopy Database (EMDB) and the Protein Data Bank (PDB) with accession numbers: EMD-60608 and 9IIX for the overall structure of Ggust-coupled TAS2R14 complex; EMD-60607 and 9IIW for the refined structure of TAS2R14 from the Ggust-coupled complex; EMD-60626 and 9IJ9 for the overall structure of Gi-coupled TAS2R14 complex; EMD-60627 and 9IJA for the refined structure of TAS2R14 from the Gi-coupled complex.
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Acknowledgements
The cryo-EM data were collected at the Advanced Center for Electron Microscopy, Shanghai Institute of Materia Medica. The authors thank the staff at the Advanced Center for Electron Microscopy for their technical support. This work was partially supported by the National Natural Science Foundation of China (82373881 to J.D., 32130022 and 82121005 to H.E.X.); Young Elite Scientists Sponsorship Program by CAST (2022QNRC001 to J.D.); Shanghai Sailing Program (23YF1456800 to J.D.); the Youth Innovation Promotion Association of Chinese Academy of Sciences (to J.D.); Ministry of Science and Technology (China) grants (2018YFA0507002 to H.E.X.); Shanghai Municipal Science and Technology Major Project (2019SHZDZX02 to H.E.X.); Shanghai Municipal Science and Technology Major Project (H.E.X.); CAS Strategic Priority Research Program (XDB37030103 to H.E.X.); Shanghai Pujiang Program (23PJ1415200 to Y.L.).
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L.T. designed and screened expression constructs, optimized purification conditions for protein complexes, prepared protein samples and cryo-EM grids, and contributed to figure preparation; D.W. and F.Z. conducted cell-based functional assays and participated in figure preparation; Q.Y. performed cryo-EM data calculations, model building, and contributed to figure preparation; Y.Z. synthesized Cpd 28.1; T.D. and S.S. participated in functional studies and protein purification; J.D. initiated the project and established collaborations with Y.L., D.Y., and H.E.X.; J.D. wrote the manuscript and prepared the figures with input from all authors; Y.L., D.Y., and H.E.X. participated in manuscript editing.
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Tao, L., Wang, D., Yuan, Q. et al. Bitter taste receptor TAS2R14 activation and G protein assembly by an intracellular agonist. Cell Res 34, 735–738 (2024). https://doi.org/10.1038/s41422-024-00995-4
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DOI: https://doi.org/10.1038/s41422-024-00995-4
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