Fig. 1: FUST1 undergoes heat-dependent condensation in vivo and in vitro. | Cell Research

Fig. 1: FUST1 undergoes heat-dependent condensation in vivo and in vitro.

From: A thermosensor FUST1 primes heat-induced stress granule formation via biomolecular condensation in Arabidopsis

Fig. 1

a Time-lapse imaging of FUST1-mVenus condensation in Arabidopsis root tip cells. Scale bar, 5 μm. b FRAP of FUST1 condensates in Arabidopsis root tip cells. Time 0 s indicates the time of the photobleaching pulse. White dashed circles indicate the bleached condensates. Scale bar, 1 μm. c Plot showing the fluorescence recovery of FUST1 condensates after photobleaching in Arabidopsis root tip cells. Error bars indicate mean ± SD (n = 13). d In vitro phase separation of 2.5 μM FUST1-GFP at varying temperatures in 40 mM Tris-HCl, pH 7.4 and 100 mM NaCl. Scale bars, 5 μm. e Time-lapse imaging of 2.5 μM FUST1-GFP phase separation at 4 °C and 37 °C in 40 mM Tris-HCl, pH 7.4 and 100 mM NaCl in vitro. Scale bars, 5 μm. f FRAP of FUST1-GFP droplet. Time 0 s indicates the time of the photobleaching pulse. Scale bar, 2 μm. g Plot showing the recovery after photobleaching of FUST1-GFP droplets. Error bars indicate mean ± SD (n = 6). h Relaxation of two FUST1-GFP droplets into one droplet. Time points are indicated above. Scale bar, 2 μm.

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