Fig. 4: FUST1 interacts with and localizes in the SGs.

a The integral SG components enriched by FUST1-TurboID proximity labeling. Error bars indicate mean ± SD (n = 4). b Volcano plot showing the enrichment of proteins by IP-MS of FUST1 in Arabidopsis. The values were calculated from 4 biological replicates. c Co-immunoprecipitation of FUST1 with indicated proteins expressed in tobacco epidermal cells. Cells were treated at 37 °C for 1 h and crosslinked with UV. Empty vector was included as a negative control. d Representative confocal microscopic images of Arabidopsis root tip cells co-expressing FUST1-mScarlet with G3BP5-mVenus or PAB2-GFP under their native promoters. Roots were treated at 37 °C for 30 min. Scale bars, 10 μm. e FISH with oligodT probes in pFUST1::FUST1-mVenus/fust1-1 roots treated at 37 °C for 30 min. Scale bar, 10 μm. f Partitioning of 2.5 μM indicated proteins by 2.5 μM FUST1 droplets in 40 mM Tris-HCl pH 7.4 and 100 mM NaCl in vitro. Scale bars, 5 μm.