Fig. 5: FUST1 condensation is required for SG formation.

a Confocal microscopy images of Arabidopsis root tip cells expressing indicated proteins under the corresponding native promoters. The roots were treated as indicated. Scale bars, 10 μm. b Representative confocal microscopic images of Arabidopsis root tip cells co-expressing FUST1-mScarlet with G3BP5-mVenus or PAB2-GFP under their native promoters. Roots were treated at 33 °C for 90 min. Scale bars, 10 μm. c Time-lapse imaging of Arabidopsis root tip cells co-expressing G3BP5-mVenus or PAB2-GFP with FUST1-mScarlet under their corresponding native promoters. The plants were treated at 37 °C for the indicated time. Scale bars, 1 μm. d, e Left, Confocal microscopic images of Arabidopsis root tip cells expressing indicated proteins under corresponding native promoters in FUST1 wild type background or fust1-2 mutants. The roots were treated at 37 °C for the indicated time. Scale bars, 10 μm. Right, Statistical analysis of the number of condensates per cell in the indicated genotypes. Error bars indicate mean ± SD (n = 15). P values were calculated using two-sided Student’s t-test. ****P < 0.0001. f FISH with Cy5-oligodT probes in Col-0 and fust1 mutants treated at 37 °C for 30 min. Scale bar, 5 μm. P values were calculated using two-sided Student’s t-test. ****P < 0.0001.