Fig. 2: Identification of gene mutations in monoclonal expansion of J∆NI8 vectors. | Gene Therapy

Fig. 2: Identification of gene mutations in monoclonal expansion of J∆NI8 vectors.

From: Genetic mutations in HSV-1 replication-defective vectors: Implications for their safety in gene therapy applications

Fig. 2

A The upper part represents a schematic representation of the HSV-1 genome (KOS strain) and J∆NI8 recombinant vector in which a Gene of Interest (GOI, e.g., tdTomato) can be inserted through Gateway cloning. The bottom part illustrates the two clones of J∆NI8 viral vectors. B Kraken 2 classifications summary, showing the percentage of reads falling into the top 5 taxa across different ranks. C IGV snapshot of BigWig file showing the DNA coverage across the HSV-1 Kos genome strain. Each sample auto-scaled individually. D Variant calling summary with different detection sensitivity parameters. E Identified mutation in the UL27 gene (glycoprotein B; gB) in the monoclonal expanded J∆NI8 batches. Mutations common to the two batches are boxed in pink, those identified in a single one (J∆NI8-Syn) are in violet.

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