Fig. 3: Dye and electrical coupling of neurons transduced with the J∆NI8-1 vector. | Gene Therapy

Fig. 3: Dye and electrical coupling of neurons transduced with the J∆NI8-1 vector.

From: Genetic mutations in HSV-1 replication-defective vectors: Implications for their safety in gene therapy applications

Fig. 3

A Representative sequential images showing green fluorescence monitoring at different time points during voltage-clamp recording of a J∆NI8-1 transduced neuron using a Neurobiotin-488 loaded pipette. Note the dye diffusing through the cytoplasm of the patched cell; asterisks represent the non-specific labeling of dead cells prior to patching. As shown in (B) Neurobiotin-488 can diffuse in neighboring tdTomato+ cells (arrows), but not within tdTomato- neurons (indicated by arrowheads in the bright field image in (A)). C Voltage clamp recording during the dye loading of the patched cell. Insert represents a higher magnification of the bursting activity. D Dual patch clamp recording of two J∆NI8-1 neurons demonstrating the electrical coupling of putatively fused cells. Note that only one neuron (blue) has been injected with suprathreshold depolarizing current steps, while the second (green) displays a coherent and synchronous response.

Back to article page