Fig. 4: Calcium homeostasis alteration in neurons transduced with the J∆NI8-1 vector.
A Pseudocolored images representative of calcium level measured with the Fura-2 Ca2+ dye in control and J∆NI8-1 transduced neurons at resting state. B Histograms summarizing mean intracellular calcium levels under resting conditions. Data are shown as Median with interquartile range; each dot represents a single cell. CTRL = 190, n = 258 cells of 5 independent experiments, J∆NI8-1 = 269, n = 202 cells of 5 independent experiments. Statistical significance in (B) was calculated using the Mann-Whitney U test. ***p < 0.001. C Voltage clamp recording of transduced neurons during bath application of the voltage-gated sodium channels blocker TTX (1 µM). D–E Voltage clamp recording of transduced neurons during bath application of either the T-type calcium channel blocker NNC 55-0396 (10 µM, D) or the AMPA receptor antagonist, NBQX (5 µM, E). F, G Histograms summarizing average bursting frequency and instantaneous frequency. Data are shown as Mean ± SEM. F ACSF = 5.61 ± 1.13 Hz (black), NBQX = 6.52 ± 2.46 Hz (orange), NNC = 0.20 ± 0.12 Hz (light blue) and TTX = 0 Hz (magenta). G ACSF = 17.67 ± 3.35 Hz (black), NBQX = 16.13 ± 1.95 Hz (orange), NNC = 6.63 ± 3.5 Hz (light blue) and TTX = 0 Hz (magenta). Statistical significance in F and G was calculated using the Kruskal–Wallis one-way analysis of variance followed by the Dunn’s post hoc test. *p < 0.05; *** p < 0.001.
