Fig. 5: Comparative electrophysiological analysis of the two UL27 gene variants: J∆NI8_A549I and J∆NI8_A549T. | Gene Therapy

Fig. 5: Comparative electrophysiological analysis of the two UL27 gene variants: J∆NI8_A549I and J∆NI8_A549T.

From: Genetic mutations in HSV-1 replication-defective vectors: Implications for their safety in gene therapy applications

Fig. 5

A–D Histograms summarizing average Resting Membrane Potential, Input Resistance, spontaneous event Frequency, and Instantaneous Frequency of neurons transduced with different viral batches at a multiplicity of infection of 1 (MOI = 1). Data are shown as Mean ± SEM. Every dot represents a single cell of 3 different biological replicates. A NT = -57 ± 1.3 mV, J∆NI8_A549I = −58 ± 1.5 mV, J∆NI8_A549T = -56 ± 1.9 mV, J∆NI8-1 = -65 ± 2.7 mV. B NT = 368 ± 28 MΩ, J∆NI8_A549I = 441 ± 31 MΩ, J∆NI8_A549T = 382 ± 27 MΩ, J∆NI8-1 = 97 ± 11 MΩ. C NT = 0.1 ± 0.04 Hz, J∆NI8_A549I = 0.54 ± 0.2 Hz, J∆NI8_A549T = 0.19 ± 0.07 Hz, J∆NI8-1 = 3.1 ± 0.5 Hz. D NT = 0.4 ± 0.27 Hz, J∆NI8_A549I = 1.79 ± 0.61 Hz, J∆NI8_A549T = 0.28 ± 0.1 Hz, J∆NI8-1 = 17.57 ± 2.78 Hz. Statistical significance in (A–D) was calculated using one-way ANOVA followed by Tukey’s post-test for multiple comparisons. *p < 0.05; **p < 0.01, ***p < 0.001. E–G. Histograms summarizing average Resting Membrane Potential, Input Resistance, spontaneous event Frequency, and Instantaneous Frequency of neurons transduced with vectors carrying either J∆NI8_A549I or J∆NI8_A549T at MOI 1 or 3. E NT = −53 ± 1.7 mV, J∆NI8_A549I (MOI 1) = −55 ± 3 mV, J∆NI8_A549I (MOI 3) = -64 ± 2 mV, J∆NI8_A549T (MOI 1)= −57 ± 2.6 mV, J∆NI8_A549T (MOI 3)= -58 ± 1.8 mV. F NT = 430 ± 54 MΩ, J∆NI8_A549I (MOI 1) = 231 ± 19 MΩ, J∆NI8_A549I (MOI 3) = 280 ± 27 MΩ, J∆NI8_A549T (MOI 1) = 424 ± 83 MΩ, J∆NI8_A549T (MOI 3) = 458 ± 52 MΩ. G: NT = 0.28 ± 0.11 Hz, J∆NI8_A549I (MOI 1) = 1.24 ± 0.3 Hz, J∆NI8_A549I (MOI 3) = 2.1 ± 0.5 Hz, J∆NI8_A549T (MOI 1) = 0.35 ± 0.15 Hz, J∆NI8_A549T (MOI 3) = 0.32 ± 0.08 Hz. H NT = 0.55 ± 0.27 Hz, J∆NI8_A549I (MOI 1) = 3.76 ± 1.11 Hz, J∆NI8_A549I (MOI 3) = 7.86 ± 1.7 Hz, J∆NI8_A549T (MOI 1) = 0.84 ± 0.56 Hz, J∆NI8_A549T (MOI 3) = 1.06 ± 0.31 Hz. Statistical significance in (E–G) was calculated using one-way ANOVA followed by Tukey’s post-test for multiple comparisons. *p < 0.05; **p < 0.01, ***p < 0.001. I Examples of spontaneous activity in control (black trace) and J∆NI8_A549I or J∆NI8_A549T transduced neurons at different MOI. J Schematic representation of the two gB variants analyzed.

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