Fig. 1: Ancestral G0 strain fitness.

Box plots of fitness data for the ancestral (G0) fog-2 and xol-1 control strains and the G0 ETC mutants relative to wildtype N2. Fitness is a composite measure of average daily survival and offspring production, taking into account the mean intrinsic population growth rate of the assay-specific N2 control. Each plot represents an average of 5–20 independently measured replicates. Boxes encompass the upper and lower quartiles of the data with the central line denoting the median, and upper and lower whiskers denoting the maximum and minimum excluding outliers, respectively. Single points denote statistical outliers. The horizontal line at 1.0 represents wildtype N2 fitness. From left, data for the obligately outcrossing fog-2 (green) and obligately selfing xol-1 (cream) G0 ancestral controls are shown. These are followed by groups of G0 ancestral mutants organized by ETC mutant type (left to right): the mitochondrial-encoded cox-1 (blue) and ctb-1 (orange), and the nuclear-encoded gas-1 (purple) and isp-1 (not visible). Each group encompasses three mating systems (left to right): facultatively outcrossing, obligately outcrossing (“fog-2”) and obligately selfing (“xol-1”).