Table 1 Strains, affected ETC proteins, and mating system.

From: Effects of mating system and adaptedness on the evolution of fitness and mtDNA copy number in mitonuclear mismatched C. elegans

Strain

Mitochondrial ETC Location

Mating system

N2

No ETC mutation

Facultatively outcrossing

xol-1 X

Obligately selfing

fog-2 V

Obligately outcrossing

cox-1

ETC complex IV (mtDNA)

Facultatively outcrossing

xol-1 X; cox-1 M

Obligately selfing

fog-2 V; cox-1 M

Obligately outcrossing

ctb-1

ETC complex III (mtDNA)

Facultatively outcrossing

xol-1 X; ctb-1 M

Obligately selfing

fog-2 V; ctb-1 M

Obligately outcrossing

isp-1

ETC complex III (nDNA)

Facultatively outcrossing

xol-1; isp-1

Obligately selfing

fog-2; isp-1

Obligately outcrossing

gas-1

ETC complex I (nDNA)

Facultatively outcrossing

xol-1; gas-1

Obligately selfing

fog-2; gas-1

Obligately outcrossing

isp-1 IV; ctb-1 M

ETC complex III (nDNA) & complex III (mtDNA)

Facultatively outcrossing

  1. We utilized strains containing each of four ETC mutations (two mtDNA-encoded, and two nDNA-encoded) on three genetic backgrounds affecting mating system: wildtype (facultatively outcrossing), xol-1 (obligately selfing), and fog-2 (obligately outcrossing). For logistical reasons, we also only studied a facultatively outcrossing version of the mitochondrial-nuclear double mutant, isp-1 V; ctb-1 M. Following standard C. elegans genetic nomenclature for such double mutants, the nuclear gene name is shown first alongside the chromosome number, followed by the mitochondrial gene name and “M” to denote the mitochondrial chromosome.
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