Fig. 4

Effect of hypobaric hypoxia exposure on mRNA expression and the protein levels of DDAH-2, CBS, and arginase type II. A Quantitative PCR of DDAH-2 in hypoxia-induced hypertensive and nonhypertensive rats before exposure to hypoxia (white bar) and 30 days after exposure (black bar) (n = 6 per group, *p < 0.001 and **p < 0.001). B Quantitative PCR of CBS mRNA expression in hypoxia-induced hypertensive and nonhypertensive rats before exposure to hypoxia (white bar) and after 30 days of hypoxia exposure (black bar) (n = 6 per group, *p < 0.001). (C) Quantitative PCR of arginase type II mRNA expression in hypoxia-induced hypertensive and nonhypertensive rats before exposure to hypoxia (white bar) and after 30 days of exposure to hypoxia (black bar) (n = 6 per group, *p < 0.001). Measurements were made in aortic artery tissue homogenates. The mRNA levels were normalized to GAPDH mRNA levels. The boxes correspond to Western blots analysis of aortic artery homogenates