Fig. 3: Prph2^Y141C/+/Rho^+/- and Prph2^K153∆/+/Rho^+/- exhibit improved OS morphogenesis and ultrastructure compared to their respective controls.

A Representative TEM images at low and high-magnification of tannic acid/uranyl acetate-stained retinas from WT, Rho^+/-, Prph2^Y141C/+, Prph2^Y141C/+/Rho^+/-, Prph2^K153∆/+, and Prph2^K153∆/+/Rho^+/- at P16. B Quantification of open discs at the base of the OS in the listed genotypes at P16, utilizing data from a previous publication for WT and Prph2^Y141C/+ open discs³⁷. C Quantification of OS diameters measured in the listed genotypes at P16, with each data point representing a single outer segment. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, ^∗∗∗∗P < 0.0001 by one-way ANOVA (P < 0.0001 for both open disc and OS diameter) with Tukey’s post-hoc comparison. Arrowheads highlight misaligned, overgrown, and sagittally aligned discs, as well as whorl-like structures commonly observed in Prph2^Y141C/+ and Prph2^K153∆/+ ROSs. N (number of independent eyes used for analyses) values for (B): WT: 111, Rho^+/-: 112; Prph2^Y141C/+: 152; Prph2^Y141C/+/Rho^+/-: 105; Prph2^K153∆/+: 98, Prph2^K153∆/+/Rho^+/-: 43 and for C: WT: 86, Rho^+/-: 138, Prph2^Y141C/+: 80, Prph2^Y141C/+/Rho^+/-: 139, Prph2^K153∆/+: 63, Prph2^K153∆/+/Rho^+/-: 90. Scale bar, 1 µm. Error bars represent mean ± SD.