Fig. 4: Type A and B product formation in N. benthamiana leaves. | Nature Communications

Fig. 4: Type A and B product formation in N. benthamiana leaves.

From: Regiodivergent biosynthesis of bridged bicyclononanes

Fig. 4

The HsCPTa and HsCPTb genes were transiently expressed in N. benthamiana leaves, and transgenic disk sections were incubated with the acceptor substrate grandone 1. a, b Extracted ion chromatograms (m/z [M–H]– = 501.3010 ± 0.005) confirmed the formation of 7-epi-nemorosone 3 and 7-epi-clusianone 5 in leaves expressing HsCPTa and HsCPTb, respectively. c Neither product was observed in leaves infiltrated with the GFP-encoding control construct. d Authentic reference compounds.

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