Fig. 6: Mutagenic transformation of HsCPTa into HsCPTb. | Nature Communications

Fig. 6: Mutagenic transformation of HsCPTa into HsCPTb.

From: Regiodivergent biosynthesis of bridged bicyclononanes

Fig. 6

a Progressive C-terminal exchange of HsCPTb residues with corresponding fragments of HsCPTa identified a 96 amino acids region essential for regiospecificity (box). b Sequence alignment of this region of interest, its location in the predicted HsCPTa fold (opaque model), and its interface to the central cavity (grey surface). c Progressive conversion of the HsCPTa regiospecificity into the HsCPTb regiospecificity using expanding sets of amino acid substitutions. Total amounts of type A and B products obtained in single standardised reactions are indicated. Data are means ± SD of three biological replicates.

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