Fig. 1: Identification of Tof4b and the validation of E1Lb underlying Tof4b. | Nature Communications

Fig. 1: Identification of Tof4b and the validation of E1Lb underlying Tof4b.

From: Subfunctionalisation and self-repression of duplicated E1 homologues finetunes soybean flowering and adaptation

Fig. 1

a Delimitation of the Tof4b locus to an 8512-kb region on chromosome 4 in a segregating, heterozygous inbred family from a cross between Williams 82 (Wm82) and Dongnong 50 (DN50). Flowering time was recorded in 2020 in Harbin under natural long-day conditions. Segregation of flowering time is shown in box-plot format, where the interquartile region, median and range are represented by the box, the bold vertical line and the horizontal line, respectively. n represents the number of plants. b Schematic of allelic variation in the Tof4b candidate gene E1Lb in Wm82 and DN50. Numbers refer to the nucleotide-residue position relative to the annotated translational start site. c Expression of E1Lb in the Tof4b NILs (Near Isogenic Lines). Data are the mean ± S.D. of n = 3 biological replicates and relative expression was normalised to Tubulin (Glyma.05G157300). ZT: Zeitgeber time. d Schematic of the constructs used for a transient reporter-gene assay to validate the causal mutations of E1Lb. P1 represents the promoter of Wm82, P4 represents the promoter of DN50, P2 and P3 are the transitional form. e Relative LUC activity driven by the promoters in (d). Data are the mean ± S.D. of n = 5 biological replicates. The two-sided Student’s t-test was used to generate the P values. f Phenotypes of wild-type c.v. Wm82 and tof4bCR-Wm82 plants cultivated under long-day conditions in a growth chamber. Scale bar = 10 cm. g Flowering time of Wm82 and tof4bCR-Wm82 plants grown as described in (f). Data are the mean ± S.D. of n = 10 plants. DAE Days after emergence. The two-sided Student’s t-test was used to generate the P values. h Phenotypes of wild-type c.v. DN50 and tof4bCR-DN50 plants cultivated under long-day conditions in a growth chamber. Scale bar = 10 cm. i Flowering time of DN50 and tof4bCR-DN50 plants grown as described in (h). Data are the mean ± S.D. of n = 10 plants. The two-sided Student’s t-test was used to generate the P values. Source data are provided as a Source Data file.

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