Fig. 2: Tof4b is up-regulated by E3/E4 and E2 to directly represses the expression of major florigens FT2a and FT5a. | Nature Communications

Fig. 2: Tof4b is up-regulated by E3/E4 and E2 to directly represses the expression of major florigens FT2a and FT5a.

From: Subfunctionalisation and self-repression of duplicated E1 homologues finetunes soybean flowering and adaptation

Fig. 2

Diurnal variation in Tof4b expression levels in NILs of E3/E4 (a) and NILs of E2 (b) under long-day photoperiod in a growth chamber. Data are the mean ± S.D. of n = 3 biological replicates and relative expression was normalised to Tubulin. Diurnal variation in FT2a (c) and FT5a (d) expression levels in Wm82 and Wm82-tof4bCR-Wm82 under long-day photoperiod in a growth chamber. Data are the mean ± S.D. of n = 3 biological replicates and expression was normalised to Tubulin. ChIP–qPCR assay of Tof4b–3xFLAG enrichment at the promoter region of FT2a (e) and FT5a (f). ELF1b was used as negative control. Data are the mean ± S.D. of n = 4 biological replicates. The two-sided Student’s t-test was used to generate the P values. ns indicate no significant difference (P > 0.05). Source data are provided as a Source Data file.

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