Fig. 1: Physical mapping of the highly effective Pm13.
From: A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew
a Non-denaturing fluorescence in situ hybridization (ND-FISH) patterns of Chinese Spring (left) and line No. 3778 (right). Chromosomes of Chinese Spring and No. 3778 were stained with 4’,6-diamidino-2-phenylindole (DAPI) (blue), Oligo-pSc119.2 (green), and Oligo-pTa535 (red). Orange arrows indicate the translocated chromosomes 3SlS-3BS.3BL. The experiment was independently repeated three times with consistent results. Scale bar = 10 μm. b Representative images showing phenotypic effects of Pm13 against 8 out of 108 tested Bgt isolates. Line No. 3778 carrying Pm13, YM18 carrying Pm21, and Chancellor (CC) without any Pm gene were inoculated with the indicated isolates. Leaves are shown sequentially from left to right. c Representative images showing powdery mildew symptoms on susceptible γ-ray irradiation-induced M3 mutants (IT4), the resistant parent line No. 3778 carrying Pm13 (IT0), and the susceptible control XM44 (IT4). Powdery mildew responses of mutants were examined at the M2 and M3 generations. d Physical mapping of Pm13 using chromosomal deletion mutant lines. Dot lines show the positions of markers and corresponding genes in the reference genome of Ae. longissima accession AEG-6782-2. The red marker was developed from the Pm13 candidate transcript NODE_25350. Black and white bars indicate the presence or absence of markers.
