Fig. 7: BRCA2MBD–MEILB2–BRME1MBD bridges between DNA molecules.
From: MEILB2-BRME1 forms a V-shaped DNA clamp upon BRCA2-binding in meiotic recombination
EMSAs analysing the ability of a, c BRCA2MBD–MEILB2–BRME1MBD and b, d BRCA2MBD–MEILB2–BRME1MBD ΔRKTK (truncation of BRME1 to remove the last four amino acids), at molecular concentrations indicated, to interact with a, b 75 base pair dsDNA and c, d 300 base pair dsDNA, at concentrations of 50 μM per base pair. EMSAs analysing the ability of BRCA2MBD–MEILB2–BRME1MBD harbouring e BRME1 R540E R549E and f, g MEILB2 K26E mutations, at molecular concentrations indicated, to interact with e, g 300 base pair dsDNA and f 75 base pair dsDNA, at concentrations of 50 μM per base pair. a–g Representative of three independent experiments. h Atomic force microscopy images of dsDNA (linearised plasmid) in isolation (left) and upon binding by BRCA2MBD–MEILB2–BRME1MBD complex (right); representative of three independent experiments. Source data are provided as a Source Data file.
