Fig. 8: Model of BRCA2MBD-induced dimerisation of MEILB2–BRME1MBD into a V-shaped DNA clamp.
From: MEILB2-BRME1 forms a V-shaped DNA clamp upon BRCA2-binding in meiotic recombination
MEILB2–BRME1MBD 2:2 complexes bind to DNA via their N-terminal β-caps. BRCA2MBD-binding induces dimerisation of MEILB2–BRME1MBD 2:2 complexes into a V-shaped 2:4:4 assembly, in which β-caps are physically separated by 25 nm and can clamp together DNA molecules. We propose that this could bridge between homologous chromosomes. Within the context of full-length molecules, a BRCA2-induced MEILB2–BRME1 DNA clamp may act in concert with the proximal DNA-binding domain and RAD51-/DMC1-binding regions of BRCA2, in a coordinated assembly to fulfil the specialised requirements of meiotic recombination.
