Fig. 4: Comparative Proteomic Analysis between Wild-Type Strain and ΔhmwC under Fv and Mh Conditions.

Heatmap (a) and statistics plot (b) of the proteins with different abundances between the different samples under Fv and Mh conditions (n = 3 biologically replicates). Proteins with a p value ≤ 0.05 and fold change (FC) ≥ 1.5 were considered to be up-regulated. p values were calculated by two-tailed Student’s t test. c Venn diagram displaying the proteins with differential abundance between ΔhmwC and WT under Fv or Mh conditions. d Volcano plot displaying the regulation of proteins with different abundances between ΔhmwC and WT under Fv and Mh conditions. Red and blue dots, proteins with significantly up or down differences. Several important global regulators or virulence factors were labeled with protein names. e KEGG pathway enrichment analysis of proteins with different abundances between ΔhmwC and WT under Fv and Mh. The barplot shows the significance of the p values (color) and the number of proteins (length) in the enriched KEGG pathways. p values calculated by cumulative hypergeometric test.