Fig. 3: Interhemispheric connectivity is unidirectionally blocked by DREADDs.

A, D Averaged time-frequencygram of GC from the contralesional to ipsilesional PMd before (left figure) and after (middle figure) DCZ administration and their difference (right) in the early recovery stage of Monkey A (A) and Monkey H (D). Time 0 (black line) indicates movement onset. Red rectangles indicate the time frequency ROI that was used for the calculations shown in (B, C, E–I). Black triangles at the bottom indicate the onset of grasping. B, E Anatomical dimension of GC from the contralesional to ipsilesional PMd (7 × 7 channels, encircled in red lines) in the low-frequency band around movement onset (ROI shown in A, D), before (left) and after (middle) DCZ administration and their difference (right). Each arrow indicates the directionality and strength of GC. C, F Anatomical dimension of GC from the ipsilesional to contralesional PMd. G–I Averaged value of GC in the ROI (shown in A, D) in the early recovery stage of both monkeys (A, H). Each line plot indicates the result of each experimental day in the early recovery stage (n = 9 days). Error bars indicate standard deviation. *P < 0.05 (two-sided Wilcoxon sign-rank test; P = 0.0039 [left], 0.07 [middle], 0.25 [right]).