Fig. 5: PF4 macrophages are accompanied by worse survival and less CD8+T-cell infiltration and killing capacity. | Nature Communications

Fig. 5: PF4 macrophages are accompanied by worse survival and less CD8+T-cell infiltration and killing capacity.

From: ITGB6 modulates resistance to anti-CD276 therapy in head and neck cancer by promoting PF4+ macrophage infiltration

Fig. 5

A Kaplan–Meier curve depicting the overall survival (OS) of patients with HNSCC (TCGA-Cohort) in the model group based on PF4 signature (n = 259 in PF4_low and n = 260 in PF4_high). P values were calculated by log-rank test. B Kaplan–Meier curve depicting the overall survival (OS) of patients with Pan-Cancer (TCGA-Cohort) in the model group based on PF4 signature. P values were calculated by log-rank test (n = 4892 in PF4_low and n = 4892 in PF4_high). C Kaplan–Meier curve depicting the overall survival (OS) of patients with Immunotherapy-treated renal cancer (GEO-Cohort) in the model group based on PF4 signature (n = 155 in PF4_low and n = 156 in PF4_high)55. P values were calculated by log-rank test. D Representative images of PF4 IHC staining in PF4_low (upper) and PF4_high (lower) (left) groups with HNSCC (FAH-SYSU-Cohort) (n = 40 in PF4_low and n = 41 in PF4_high). Scale bar, 100 μm. Kaplan–Meier curve depicting the overall survival (OS) of patients with HNSCC (FAH-SYSU-Cohort) in the model group based on PF4 expression (right). P values were calculated by log-rank test. E The experimental design of the HNSCC tumorigenesis model and treatment strategy (left) and representative image of PF4 IHC staining in CTL (upper) and DTR (down) groups (right). F Representative image of 4NQO-induced HNSCC in different groups (n = 6 mice). Scale bar, 1 mm. G Display of histological characteristics of HNSCC in different groups by H&E (n = 6 mice). Scale bar, 100 μm. H, I Quantification of tongue lesion area (H) and SCC number (I) in different treatment groups. Data are shown as mean ± SD (n = 6 mice). P values were calculated by one-way ANOVA with Tukey’s multiple comparison test. J Representative images of ITGB6 IHC staining in ITGB6_low (left) and ITGB6_high (right) groups with HNSCC (FAH-SYSU-Cohort) (n = 81 biological replicates). Scale bar, 100 μm. K Representative images of CD276 IHC staining in CD276_low (upper) and CD276_high (down) groups with HNSCC (FAH-SYSU-Cohort) (n = 81 biological replicates). Scale bar, 100 μm. L–N Correlation of PF4 IHC score with ITGB6 IHC score (L) and CD276 IHC score (M) (n = 81 for each group). And the correlation of ITGB6 IHC score with CD276 IHC score (N) (n = 81 for each group). Data are presented as scatter plots with a linear regression line. The shaded area represents the 95% confidence interval. Pearson correlation coefficients (R values) were calculated to assess the linear relationship between the variables. P values are exact and two-sided. Source data and exact p values are provided as a Source Data file.

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