Fig. 5: LATS1 and LATS2 modulate EBOV infectivity and egress.

a Schematic diagram of the live EBOV-GFP infection protocol. b, c Representative images and quantification of EBOV-GFP (MOI = 0.05) infected WT, LATS1-KO, LATS2-KO, and LATS1/2-dKO cells at 24- (b) and 48- (c) h.p.i. The infection efficiency was determined by a ratio of GFP-positive cells to total cell nuclei. d, e Representative images and quantification (data was normalized to the infection efficiency in b and c, respectively) of Vero cells infected with the supernatants isolated from WT, LATS1-KO, LATS2-KO, and LATS1/2-dKO cells in panels (b) and (c). Scale bar = 200 µm. f Schematic diagram of the EBOV minigenome assay. g–i EBOV minigenome transcription determined by quantification of eGFP reporter (g) or luciferase reporter (h) in WT, LATS1-KO, LATS2-KO, and LATS1/2-dKO cells. Detection of the indicated viral RNP proteins in HEK 293 cells expressing the EBOV minigenome system (i). Three times experiments were repeated independently with similar results. Data in (b–e, n = 7) and (h, n = 10) are presented as mean ± SD. Statistical analyses were performed by one-way ANOVA. Source data are provided as a Source Data file.