Fig. 7: Neural crest territory has a high rate of non-apical mitoses before the onset of EMT. | Nature Communications

Fig. 7: Neural crest territory has a high rate of non-apical mitoses before the onset of EMT.

From: Modelling variability and heterogeneity of EMT scenarios highlights nuclear positioning and protrusions as main drivers of extrusion

Fig. 7

a, b Diagrams representing a transversal section of the neural tube and how we classified apical vs non-apical mitoses. A mitosis is considered apical (green) if there are no other nuclei in between that mitotic figure and the apical domain. It is considered non-apical (magenta) if at least one nucleus separates the mitotic nucleus from the apical side. c–h Quantification of non-apical mitoses, as a percentage per embryo, in neural crest cells and the adjacent neural tube at the level of pre-EMT cephalic neural crest cells (c–e) and at the levels of pre-EMT (neural tube facing presomitic mesoderm (psm), j), early EMT (i) and late EMT (h) trunk neural crest cells. The statistical tests used are two-tailed unpaired t-test with Welch’s correction (d, n = 7 embryos per condition, p = 0.0182), one-way ANOVA/Fisher’s LSD test (g, nembryos = 12 (pre-EMT/psm level, p = 0.9796), 6 (onset of EMT/−1 to-6 somites, p = 0.0002), 7 (EMT/−7to-12 somites, p < 0.0001); NTemt/NTpsm, p = 0.002). EMT epithelial-mesenchymal transition, pH3 phosphor-histone H3. Source data are provided as a Source Data file.

Back to article page