Fig. 7: Firrm and Fignl1 deletions restore DMC1 loading in Swsap1−/− spermatocytes. | Nature Communications

Fig. 7: Firrm and Fignl1 deletions restore DMC1 loading in Swsap1−/− spermatocytes.

From: FIGNL1-FIRRM is essential for meiotic recombination and prevents DNA damage-independent RAD51 and DMC1 loading

Fig. 7

a Spreads of control, Firrm cKO, Swsap1−/− Firrm cKO and Swsap1−/− early zygotene spermatocytes stained for SYCP3 (gray), RAD51 (yellow) and RPA2 (magenta). Scale bar, 10 µm. Total numbers of on-axis RPA2 (b) or DMC1 (c) foci, or number of on-axis RPA2 foci colocalized with on-axis DMC1 foci (corrected for the numbers expected by chance) (d) in spreads from control (gray, n = 3 mice), Fignl1 cKO (orange, n = 1), Swsap1−/− Fignl1 cKO (pink, n = 1), Swsap1−/− Firrm cKO (purple, n = 1) and Swsap1−/− (blue, n = 2) spermatocytes from 17 dpp mice. Source data are provided as a Source Data file.

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