Fig. 7: Flow-based SLEC on the chip for nucleolus spectral fingerprinting.
From: Single-cell laser emitting cytometry for label-free nucleolus fingerprinting
a Configuration of the SLEC chip mounted on the microscope system and the schematic of the flow cytometry. Scale bar: 20 μm. b Four main parameters extracted from the nucleolus laser spectrum. c Phase contrast images of the suspension cells K562 and the Caco−2 cells in the suspension state. Scale bar: 20 μm. d Nucleolus laser spectral fingerprints in laser peak number, average laser peak intensity, laser peak intensity variance, and low intensity (<3e3) percentage. (A549: n = 1121, K562: n = 1182, Caco−2: n = 1276, C2C12: n = 1227). e t-SNE visualization of the laser spectral data of nucleolus. Source data are provided as a Source Data file.
