Fig. 2: Composition of eukaryotic RNA and proteins.

Relative abundance of transcript reads assigned to open reading frame (ORF) predicted proteins (a), and exclusive proteomic spectral counts assigned to ORFs (b) within the eukaryotes. Each row represents a station and depth (“7_700” = St. 7700 m). Only microeukaryote transcripts having a EUKulele taxonomic annotation are shown. Reads associated with unannotated ORFs are not shown. Note that 30 m and 115 m surface samples at BATS (St. 1) were collected using McLane pumps instead of Clio, with separate 0.2–3 μm (“0.2”) and 3–51 μm (“3”) filter fractions. NA represent microbes with lineage-conflicted taxonomic assignments and are unable to be classified at the eukaryotic supergroup level. c The relative abundance of taxonomically annotated eukaryotic ORFs contained in the translated metatranscriptome database is displayed as a single stacked barplot.