Fig. 2: Bt spores germinate preferentially in Drosophila posterior midgut.
From: Ingestion of Bacillus cereus spores dampens the immune response to favor bacterial persistence
a Time-lapse images of SA-11R/G spores during germination. b Monitoring of SA-11R/G germination in vivo in Drosophila midgut 4 h post-spore-ingestion. Insets show enlargement of a single focal plan. Note that anteriorly GFP (i.e. germinating spores) are barely detectable. c Plots of the average fluorescence intensity (represented as mean gray value) of SA-11R/G germination measured along the Drosophila midgut presented in b. For all plot analyses of average fluorescence shown in this paper, the red line represents the average of the spore fluorescence (NHS-ester Alexa546) and the green line represents the average fluorescence of vegetative cells (GFP). d, e Bacterial load of Btk (SA-11) or Bc (ATCC 14579) in anterior (d) and posterior (e) Drosophila midguts. Green bars (non-heated samples) represent the whole Btk or Bc bacterial loads (spores and vegetative cells). Red bars (heated samples) represent the proportion of spore loads. Data represent the mean ± SEM of at least five independent experiments. Each experiment corresponds to five split midgut. Source data are provided as a Source Data file.
