Fig. 2: Mass Spec data for compound 1 binding to IDH1^wt and IDH1 ^R132H and substrate displacement.

a Compound 1 titration showing (blue) binding to IDH1^R132H that is unaffected by the addition of 10 mM ICT (red). b Compound 1 titration showing (blue) binding to IDH1^wt that is decreased by the addition of 10 mM ICT (red). c Percent change of compound 1 MS response when binding to IDH1^R132H or IDH1^wt in the presence of 10 mM ICT or aKG, when compared to compound 1 MS response for binding to apo-proteins. The above MS binding data confirms compound 1 binds both apo-IDH1^wt and apo-IDH1 ^R132 but shows that compound 1 binding is affected by substrate binding only for IDH1^wt and not mutant IDH1 ^R132.