Fig. 3: Viral and cellular proteins that bind to ER-I G4.

a Scheme for the pull-down assay to identify viral and cellular proteins binding to ER-I G4 (see Methods). b, c HF cells were infected with HCMV at an MOI of 1. Cell lysates were prepared at 120 h and used for pull-down assays. Biotinylated ODNs (0.5 μg), pre-incubated to form G4 or i-motif structures (see the Methods), were incubated with 50 μg of cell lysates and immobilized on streptavidin beads. After washing, the proteins were eluted and analyzed by immunoblotting with specific antibodies for hnRNP K, IE1/IE2, p52 (encoded by UL44), and UL84. Input (1/10), 5 μg of cell lysates. (-), no biotinylated ODNs added (b). The relative binding efficiency of proteins [input (1/10)%] to different ODNs in three independent experiments is also indicated in (c). The data are shown as the mean ± SD. Source data are provided as a Source Data file.