Fig. 3: Nuclear MTHFD2 interacts with key cell division factors.
From: Nuclear localization of MTHFD2 is required for correct mitosis progression
a Volcano plot of top nuclear MTHFD2 interactors identified in HCT116 cells. Interactors with a log2 fold change >= 2.3 and Bonferroni False Discovery Rate (BFDR) <= 0.2 are colored according to their functional category. IP, immunoprecipitation. b Network of top nuclear MTHFD2 interactors. The color indicates the functional category, the size represents the fold change and the width of the edges shows the interaction score. c Biological Process gene ontologies enriched in MTHFD2 core co-expressed genes. d One-carbon folate metabolism pathway with enzymes colored by the enrichment of mitotic terms in the Gene Ontology enrichment analysis performed with their core co-expressed genes. Gray indicates the absence of data. DHF, dihydrofolate; THF, tetrahydrofolate. Scheme adapted from Lin et al. 4. Two-dimensional density plots of 5% top cells with the highest nuclear (e, g) or cytosolic (f, h) MTHFD2 signal obtained from the adapted FUCCI4 MCF7 (e, f) and U2OS (g, h) cells along with the cell cycle phase; turquoise and clover mean intensities (x and y axis, respectively) are in log10 scale.
