Fig. 6: Nuclear MTHFD2 is sufficient for mitosis progression.
From: Nuclear localization of MTHFD2 is required for correct mitosis progression
a Percentage of mitotic cells in HCT116 MTHFD2 wild-type (WT), nuclear (NLS), and knock-out (KO1, KO2) conditions; means + s.d. (n = 3), unpaired two-tailed t-test. b Percentage of WT, NLS and KO1 cells at different mitotic phases; means + s.d. (n = 3), a minimum of 100 mitotic cells per replicate were analyzed, unpaired two-tailed t-test. c Quantification of anaphase bridges in WT, NLS and KO1 cells; means + s.d. (n = 3), a minimum of 10 anaphases per replicate were analyzed, unpaired two-tailed t-test. d Comparison of the log2 mean intensity of nuclear levels of H4K20me1 in WT, NLS, KO1 and KO2 cells; unpaired two-tailed Wilcoxon test. Representative images are shown on the right; non-confocal mode, scale bar 10 μm. Source data are provided as a Source Data file. The n indicates the number of biological sample replicates for a–c; and the number of individual cells for d, where 3 biological replicates were pooled.
