Table 1 Bacterial strains used in this study
From: Bacterial cell surface characterization by phage display coupled to high-throughput sequencing
Strain no. | Background | Genotype | Notes | Source or Reference |
|---|---|---|---|---|
Pseudomonas aeruginosa | ||||
220 | PAO1 | wild-type | J. Engel | |
1468, PAO397 | PAO1 | Δ(mexAB-oprM) nfxB Δ(mexCD-oprJ) Δ(mexJKL) Δ(mexXY) OpmH+ ΔopmH362 Δ(mexEF-oprN) | deletion of five efflux systems and outer membrane porins; “Δefflux” | |
1469 | PAO397 | attB::(PBAD-mexAB-oprM) | efflux mutant with mexAB oprM expressed in trans from chromosome | S. Lory |
1470 | PAO397 | attB::(PBAD-mexCD-oprJ) | efflux mutant with mexCD oprJ expressed in trans from chromosome | S. Lory |
1471 | PAO397 | attB::(PBAD-mexEF-oprN) | efflux mutant with mexEF oprN expressed in trans from chromosome | S. Lory |
1472 | PAO397 | attB::(PBAD-mexJK-oprM) | efflux mutant with mexJK oprM expressed in trans from chromosome | S. Lory |
1473 | PAO397 | attB::(PBAD-mexXY-oprM) | efflux mutant with mexXY oprM expressed in trans from chromosome | S. Lory |
1469 | PAO397 | mpMQ72::(PBAD-mexAB-oprM) | efflux mutant with mexAB oprM expressed in trans from high-copy plasmid | This study |
1470 | PAO397 | mpMQ72::(PBAD-mexCD-oprJ) | efflux mutant with mexCD oprJ expressed in trans from high-copy plasmid | This study |
1471 | PAO397 | mpMQ72::(PBAD-mexEF-oprN) | efflux mutant with mexEF oprN expressed in trans from high-copy plasmid | This study |
1472 | PAO397 | mpMQ72::(PBAD-mexJK-oprM) | efflux mutant with mexJK oprM expressed in trans from high-copy plasmid | This study |
1473 | PAO397 | mpMQ72::(PBAD-mexXY-oprM) | efflux mutant with mexXY oprM expressed in trans from high-copy plasmid | This study |
517 | PAK | wild-type | J. Mattick | |
822 | PAK | ΔflhA | does not produce flagellum | |
388 | PAK | ΔfliC | produces flagellar hook-basal body but not filament | J. Mattick |
1330 | PAK | ΔfliC ΔfleN | produces multiple flagellar hook-basal body assemblies but no filaments | |
1299 | PAK | ΔfleN | produces multiple flagella | |
380 | PAK | ΔpilA | does not produce the type IV pilus | |
311 | PAO1 | ∆pilA | does not produce the type IV pilus | |
209 | PA103 | wild-type | ||
430 | PA14 | wild-type | F. Ausubel | |
Escherichia coli | ||||
n/a | BL21(DE3) | F- ompT hsdSB (rB-, mB-) gal dcm (DE3) | For protein expression | Invitrogen |
n/a | OmniMAX™ 2 T1R | F´ {proAB lacIq lacZΔM15 Tn10(TetR) Δ(ccdAB)} mcrA Δ(mrr hsdRMS-mcrBC) Φ80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD | Phage display host strain | Invitrogen |
n/a | DH5α | F- Φ80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK-, mK + ) phoA supE44 λ- thi-1 gyrA96 relA1 | Used for subcloning | Invitrogen |
