Table 3 Primers used in this study
From: Bacterial cell surface characterization by phage display coupled to high-throughput sequencing
Name | Description | Sequence | Ref |
|---|---|---|---|
Al.CH2 | cDNA synthesis | ATGGAGAGGACGTCCTTGGGT | |
AlCH2.2 | cDNA synthesis | TTCGGGGGGAAGAYRAAGAC | |
AlVHH-F1 | Alpaca VHH, forward | CTTGCGGCCGCTCAGKTGCAGCTCGTGGAGWCNGGNGG | |
AlVHH-shR1 | Alpaca VHH, reverse, short-hinge | GATCGGCGCGCCGAGGGGTCTTCGCTGTGGTGCG | |
AlVHH-lhR1 | Alpaca VHH, reverse, long-hinge | GATCGGCGCGCCGGTTGTGGTTTTGGTGTCTTGGG | |
pD-seq | Sanger sequencing of phage clones | TCCGGCTCGTATGTTGTGTGGAAT | This study |
CDR123-seq-F | HTS library preparation; amplifies CDR1—3; adds Illumina R1 sequencing primer binding site | tcgtcggcagcgtcagatgtgtataagagacagTCCTGTGCAGCCTC | This study |
CDR123-seq-R | HTS library preparation; amplifies CDR1—3; adds Illumina R2 sequencing primer binding site | gtctcgtgggctcggagatgtgtataagagacagACCTGGGTCCCCTG | This study |
i5-seq-F | HTS library preparation; adds i5 index sequence and Illumina P5 flow cell adapter | AATGATACGGCGACCACCGAGATCTACACnnnnnnnntcgtcggcagcgtc | This study |
i7-seq-R | HTS library preparation; adds i7 index sequence and Illumina P7 flow cell adapter | CAAGCAGAAGACGGCATACGAGATnnnnnnnngtctcgtgggctcgg | This study |
rVHH-F | resynthesized VHH fragment amplification for cloning into pCER243 | CTGTTTAGAGGCGTTCAGTCTCAGGTGCAGCTGGTGGAGTCTGGCGGAGGCCTGGTGC | This study |
rVHH-R | resynthesized VHH fragment amplification for cloning into pCER243 | TCCACCAGAGCCACCTCCGCCGGAGGAG | This study |
pD-pCER-F | cloning from pD into pCER243 | ctgtttagaggcgttcagtCTCAGTTGCAGCTCGTGGAGTCG | This study |
pD-pCER-R | cloning from pD into pCER243 | tccaccagagccacctccgcCaGAGGAGACGGTGACCTGGGTCC | This study |
