Fig. 2: The PAXT, but not the NEXT and TRAMP complexes, regulates the expression of 3XTs.
From: The MTR4/hnRNPK complex surveils aberrant polyadenylated RNAs with multiple exons
a–d (left) qRT-PCR analysis of mono- (blue) and multi-exon (red) 3XT expression in HeLa cells transfected with siRNA targeting EXOSC5 (a), PABPN1 (b), RBM7 (c) or ZCCHC7 (d). qRT-PCR results are expressed as the mean ± s.d. (n = 3 biologically independent replicates). Paired two-sided Student’s t-test; *p < 0.05. (middle) qRT-PCR analysis of EXOSC5, PABPN1 or RBM7 expression in HeLa cells transfected with siRNA targeting EXOSC5 (a), PABPN1 (b), RBM7 (c), or ZCCHC7 (d). qRT-PCR results are expressed as the mean ± s.d. (n = 3 biologically independent replicates). Paired two-sided Student’s t-test; *p < 0.05. (right) Cell lysates from HeLa cells transfected with siRNA targeting the indicated genes were subjected to immunoblotting analysis with anti-EXOSC5 (a), anti-PABPN1 (b), anti-RBM7 (c), anti-ZCCHC7 (d), or anti-GAPDH antibodies. GAPDH was used as a loading control. The exact p-values are a ATP23 3XT: p = 0.00833 (siCont vs siEXOSC5#1), p = 0.00927 (siCont vs siEXOSC5#2), TP53TG1 3XT: p = 0.00048 (siCont vs siEXOSC5#1), p = 0.00067 (siCont vs siEXOSC5#2), USP45 3XT: p = 0.00187 (siCont vs siEXOSC5#1), p = 0.00312 (siCont vs siEXOSC5#2), HECTD2 3XT: p = 0.00187 (siCont vs siEXOSC5#1), p = 0.00312 (siCont vs siEXOSC5#2), SPRED2 3XT: p = 0.00977 (siCont vs siEXOSC5#1), p = 0.02162 (siCont vs siEXOSC5#2), KCTD13 3XT: p = 0.00443 (siCont vs siEXOSC5#1), p = 0.00792 (siCont vs siEXOSC5#2), EXOSC5: p = 0.00010 (siCont vs siEXOSC5#1), p = 0.00007 (siCont vs siEXOSC5#2); b ATP23 3XT: p = 0.00054 (siCont vs siPABPN1#1), p = 0.01888 (siCont vs siPABPN1#2), TP53TG1 3XT: p = 0.02140 (siCont vs siPABPN1#1), p = 0.04520 (siCont vs siPABPN1#2), USP45 3XT: p = 0.00101 (siCont vs siPABPN1#1), p = 0.00695 (siCont vs siPABPN1#2), HECTD2 3XT: p = 0.00956 (siCont vs siPABPN1#1), p = 0.03642 (siCont vs siPABPN1#2), SPRED2 3XT: p = 0.01983 (siCont vs siPABPN1#1), p = 0.01966 (siCont vs siPABPN1#2), KCTD13 3XT: p = 0.01838 (siCont vs siPABPN1#1), p = 0.03199 (siCont vs siPABPN1#2), PABPN1: p = 0.00088 (siCont vs siPABPN1#1), p = 0.00021 (siCont vs siPABPN1#2); c RBM7: p = 0.00033 (siCont vs siRBM7#1), p = 0.00006 (siCont vs siRBM7#2); d ZCCHC7: p = 7.8721E-07 (siCont vs si ZCCHC7#1), p = 0.00009 (siCont vs si ZCCHC7#2). Source data are provided as a Source Data file.
