Fig. 4: hnRNPK recognizes multi-exon 3XRs to promote the degradation of host 3XTs.
From: The MTR4/hnRNPK complex surveils aberrant polyadenylated RNAs with multiple exons
a HeLa cells were transfected with a 3XR-fused rabbit β-globin gene and then subjected to qRT-PCR analysis. Results are expressed as the mean ± s.d. (n = 3 biologically independent replicates). Paired two-sided Student’s t-test; *p < 0.05. b Lysates from HeLa cells transfected with a 3XR-fused rabbit β-globin gene and Flag-tagged hnRNPK were subjected to immunoprecipitation with an anti-Flag antibody followed by qRT-PCR analysis to detect the indicated RNAs. SNHG19 RNA was used as a negative control. Results are expressed as the mean ± s.d. (n = 3 biologically independent replicates). Paired two-sided Student’s t-test; *p < 0.05. c Schematic representation of wild-type (WT) and deletion mutant (delKH1 ~ 3) hnRNPK proteins. KH; K homology. d Lysates from HCT116 cells transfected with a KCTD13 3XR-fused rabbit β-globin gene along with wild-type or mutant Flag-tagged hnRNPK were subjected to immunoprecipitation with an anti-Flag antibody followed by qRT-PCR analysis to detect β-globin mRNA and SNHG19 RNA. SNHG19 RNA was used as a negative control. Results are expressed as the mean ± s.d. (n = 3 biologically independent replicates). Paired two-sided Student’s t-test; *p < 0.05. The exact p-values are a β-globin: p = 0.00002 (Mock vs HECTD2 3XR), p = 0.00147 (Mock vs SPRED2 3XR), p = 0.00036 (Mock vs KCTD13 3XR); b β-globin: p = 0.01727 (Mock vs HECTD2 3XR), p = 0.03705 (Mock vs SPRED2 3XR), p = 0.00146 (Mock vs KCTD13 3XR); d β-globin: p = 0.01415 (WT vs delKH1), p = 0.01123 (WT vs delKH3). Source data are provided as a Source Data file.
