Fig. 5: KCTD13 3XT protein forms a liquid-like assembly.

a The structure of the protein derived from KCTD13 3XT is predicted by AlphaFold and the PSR region (pink) translated from the KCTD13 CDS contains a disorder region. b Detection of KeXT bodies in HeLa cells transfected with siMTR4#2. Representative maximum projection immunofluorescence image of HeLa cells transfected with siMTR4#2 and immunolabeled with KCTD13 3XT protein (orange). Nuclei were stained with Hoechst33258 (blue). Scale bar, 10 µm. This experiment was repeated independently three times with similar results. c Detection of KeXT bodies in HeLa cells transfected with Flag-tagged KCTD13 3XT protein. Immunofluorescence images of HeLa cells transfected with Flag-tagged KCTD13 3XT protein (WT) or Flag-control (Mock) and immunostained with anti-Flag antibody (orange). Nuclei were stained with Hoechst33258 (blue). Scale bar, 10 µm. This experiment was repeated independently three times with similar results. d The 3XR is required for KeXT body assembly. Immunofluorescence images of HeLa cells transfected with PSR-deletion mutant Flag-tagged KCTD13 3XT protein (del-PSR) or Flag-control (Mock) and immunostained with anti-Flag antibody (orange). Nuclei were stained with DAPI (blue). Scale bar, 10 µm. This experiment was repeated independently three times with similar results. e KeXT bodies in HeLa cells transfected with siMTR4#2 in the presence of 1,6-hexandiol or 2,5-hexandiol. Representative maximum projection immunofluorescence image of HeLa cells transfected with siMTR4#2, treated with or without 1,6-hexandiol or 2,5-hexandiol, and immunolabeled with KCTD13 3XT protein (orange). Nuclei were stained with Hoechst33258 (blue). Scale bar, 10 µm. This experiment was repeated independently three times with similar results.