Fig. 1: Pol ζ-mediated DNA synthesis is induced in response to replication stress. | Nature Communications

Fig. 1: Pol ζ-mediated DNA synthesis is induced in response to replication stress.

From: A sophisticated mechanism governs Pol ζ activity in response to replication stress

Fig. 1

Aphidicolin (APH)-insensitive EdU incorporation (a, b) and the amount of REV3L-C at active replication forks (c, d) were monitored in HCT116-R-REV3L cells upon indicated treatments. DOX, 20 ng/mL doxycycline; HU, 2 mM hydroxyurea; REV1i, 2 μM JH-RE-06. Left panel: Representative images. Scale bar, 50 μm in (a, b) 20 μm in (c, d). Right panel: Quantitative analyses results. Sample size and mean of each measurement are indicated. P values were determined using the two-sided Mann-Whitney test. Each experiment was independently replicated at least twice, yielding similar results each time. Source data are provided as a Source data file.

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