Table 1 Summary of the SPR analyses in this study

From: CD5L associates with IgM via the J chain

Chip

Ligand

Analyte

Coupling

Experiment type

Fitting model

ka (1/Ms)

kd (1/s)

Kd (nM)

CM5

Human Fcμ–J

Human CD5L

Random

Single-cycle kinetics

1:1 binding

2.8 × 105 ± 1.7 × 105

8.3 × 10−3 ± 5.6 ×  10−3

28.7 ± 5.9

CM5

Cat Fcμ–J

Cat CD5L

Random

Single-cycle kinetics

1:1 binding

1.9 × 104 ± 5.8 × 103

3.3 × 10−4 ± 9.6 × 10−5

17.9 ± 8.0

CM5

Mouse Fcμ–J

Mouse CD5L

Random

Single-cycle kinetics

1:1 binding

3.4 × 104 ± 1.0 × 104

8.2 × 10−4 ± 4.0 × 10−4

26.0 ± 12.5

SA

Human Fcμ–J

Human CD5L

Oriented

Single-cycle kinetics

1:1 binding

6.1 × 105 ± 1.9 × 105

1.1 × 10−2 ± 2.3 × 10−3

18.1 ± 3.8

SA

Cat Fcμ–J

Cat CD5L

Oriented

Single-cycle kinetics

1:1 binding

2.7 × 105 ± 3.0 × 105

2.5 × 10−3 ± 1.7 × 10−3

14.4 ± 8.4

SA

Mouse Fcμ–J

Mouse CD5L

Oriented

Single-cycle kinetics

1:1 binding

4.6 × 105 ± 4.1 × 105

1.5 × 10−2 ± 1.0 × 10−2

36.1 ± 10.3

SA

Human Fcμ–J

Human CD5L (C191S)

Oriented

Single-cycle kinetics

1:1 binding

1.1 × 106 ± 1.0 × 106

1.3 × 10−2 ± 1.0 × 10−2

13.2 ± 2.2

CM5

Fcμ–J–CD5L

FcμR

Random

Single-cycle kinetics

1:1 binding

1.1 × 107 ± 1.9 × 106

9.6 × 10−2 ± 2.0 × 10−2

8.8 ± 0.3

CM5

Fcμ–J

FcμR

Random

Single-cycle kinetics

1:1 binding

1.0 × 107 ± 2.5 × 106

7.5 × 10−2 ± 2.2 × 10−2

7.4 ± 0.4

CM5

Fcμ–J–CD5L

pIgR

Random

Single-cycle kinetics

1:1 binding

1.1 × 105 ± 8.4 × 104

1.3 × 10−2 ± 1.0 × 10−2

134 ± 11.3

CM5

Fcμ–J

pIgR

Random

Single-cycle kinetics

1:1 binding

1.4 × 105 ± 7.0 × 103

4.6 × 10−4 ± 3.3 × 10−4

3.5 ± 2.6

CM5

FcμR

Fcμ–J–CD5L

Random

Multi-cycle kinetics

1:1 binding

2.6 × 106 ± 7.6 × 105

1.1 × 10−3 ± 2.4 × 10−4

0.4 ± 0.04

CM5

FcμR

Fcμ–J

Random

Multi-cycle kinetics

1:1 binding

3.0 × 106 ± 2.0 × 106

7.1 × 10−4 ± 7.4 × 10−4

0.2 ± 0.1

SA

pIgR

Fcμ–J

Oriented

Multi-cycle kinetics

1:1 binding

1.9 × 105 ± 2.7 × 104

1.3 × 10−3 ± 3.2 × 10−4

6.9 ± 0.7

SA

pIgR

Fcμ–J–CD5L

Oriented

Multi-cycle kinetics

Two-state reaction

9.8 × 104 ± 3.1 × 103 (ka1)

4.6 × 10−3 ± 5.7 × 10−4 (ka2)

5.6 × 10−2 ± 5.2 × 10−3 (kd1)

7.2 × 10−3 ± 3.9 × 10−3 (kd2)

341 ± 102

1:1 binding

7.2 × 104 ± 1.5 × 104

1.4 × 10−2 ± 5.6 × 10−3

201 ± 118

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