Fig. 5: The utility of the tax-aliquots clustering approach is demonstrated on a simplified mock metatranscriptome, highlighting enhanced annotation at finer taxonomic resolution.
From: Missing microbial eukaryotes and misleading meta-omic conclusions
A Left panel: Workflow schematic; first, we annotated a “mock metatranscriptome” (a Phaeocystis pouchetii transcriptome) and filtered putative haptophyte sequences using EUKulele (Right panel: results of annotating the mock metatranscriptome with BLAST + LCA (EUKulele) as compared to mmseqs2). Then, we split the sequences into two parts, and annotated half of putative haptophyte sequences with a custom Phaeocystis-only reference database which excluded the half of P. pouchetii being tested (but included the other half as a simulated partial database transcriptome) using BLAST + LCA (EUKulele), mmseqs2, and tax-aliquots. B Tax-aliquots clusters using the “permissive” clustering scheme for the putative haptophyte sequences retrieved from the BLAST + LCA approach in panel B. C Comparison of the fate of the test putative haptophyte sequences between the BLAST + LCA, mmseqs2, and tax-aliquots approaches.
