Fig. 6: T.mu colonization enhances mucosal antigen-specific IgA production in a Blt1-dependent manner. | Nature Communications

Fig. 6: T.mu colonization enhances mucosal antigen-specific IgA production in a Blt1-dependent manner.

From: A mouse protozoan boosts antigen-specific mucosal IgA responses in a specific lipid metabolism- and signaling-dependent manner

Fig. 6

A Schematic of arachidonic acid lipoxygenase (Lox) pathway. B–E Groups of mice were administered once with either PBS (Ctrl) or T.mu via the oral route on day 0. From day 6 and thereafter, the mice were treated with vehicle, ML-355 (B, C), or Montelukast (D, E) once daily by oral gavage. On day 7, the mice were intraperitoneally challenged with OVA plus CFA. The relative levels of serum (B, D) and cecal content (C, E) anti-OVA IgA were determined on day 21 (n = 6 mice/group) in (B) and (C). n = 12 mice/group for Ctrl and T.mu groups in (D) and (E), and n = 6 mice/group for Ctrl+Montelukast and T.mu+Montelukast groups in (D) and (E). F, G Groups of Blt1−/− and the littermate Blt1+/− mice were orally administered once with either PBS or T.mu on day 0. On day 7, the mice were intraperitoneally challenged with OVA plus CFA. The relative levels of serum (F) and cecal content (G) anti-OVA IgA were determined on day 21 (n = 7 mice/group for Blt1+/− and Blt1+/−-T.mu groups, n = 5 mice/group for Blt1−/− and Blt1−/−-T.mu groups). Data are shown as mean ± SEM. One-way ANOVA with Tukey’s post hoc test (B–G) was performed. AU arbitrary unit. Source data are provided as a Source Data file.

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