Fig. 7: T.mu colonization enhanced the effectiveness of an oral vaccine against a mucosal pathogen.
A Schematic of the experimental setup for (A–K). B–D The relative levels of serum (B) and cecal content (C) IgA or serum IgG (D) 3 weeks after the introduction of the heat-inactivated S. Typhimurium vaccine (n = 6 mice/group). E Survival curve (n = 11 mice/group). F, G S. Typhimurium colony-forming units (CFU) in the cecal content (F) and mLN (G) 24 h post-infection (n = 6 mice/group). H, I The mice were treated intraperitoneally with vehicle or zileuton once daily from day 1. H The relative levels of serum IgA were determined 3 weeks after the first dose of vaccine. I Survival curve (n = 6 mice/group). J, K Groups of mice (n = 12 mice/group) were fed with either a control diet or an ARA-supplemented diet from day 7 and treated with heat-inactivated S. Typhimurium vaccine orally on day 0 and day 14, followed by live S. Typhimurium challenge. J The relative levels of serum IgA were determined 3 weeks after the first dose of vaccine. K Survival curve. L–N Blt1−/− mice and the littermate controls (Blt1+/− mice) were colonized with T.mu, 1 week later were vaccinated with heat-inactivated S. Typhimurium, and two more weeks later injected the second dose of vaccine. 7 days after the second vaccination, mice were challenged with live S. Typhimurium. L The colonic transcription of Il18 two days after infection was determined by qRT-PCR (n = 6 mice/group). M The fecal anti-S. Typhimurium IgA titers 8 days after the second vaccination (n = 8 mice/group). N Survival curve (n = 8 mice/group). All data are shown as mean ± SEM. Log-rank (Mantel-Cox) test (E, I, K, and N), Two-way ANOVA with Sidak’s multiple comparisons test (B and D), or One-way ANOVA with Tukey’s post hoc test (H and M), or two-sided Student’s t-test (C, F, G, J and L) were performed. AU arbitrary unit. Source data are provided as a Source Data file.
