Fig. 7: Roles of FOXM1, MAT2A and MAT2B in transforming growth factor (TGF)-β1 signaling in LX-2 cells and LPS effects in macrophages, and their presence in EVs.

a Time courses of protein expression of FOXM1, MATα2, MAT2β, SMAD3, α–SMA, and COL1A1 after TGF-β1 treatment in LX-2 cells. b FOXM1, MATα2, MAT2β, SMAD3, α-SMA and COL1A1 protein levels after siRNA knockdown of FOXM1, MAT2A, or MAT2B in LX-2 cells after 24 h. c Protein levels of FOXM1, MATα2, MAT2β, SMAD3, α-SMA, and COL1A1 after FOXM1 overexpression with or without MAT2A or MAT2B siRNA knockdown and TGF–β1 treatment (20 ng/ml) for 24 h in LX-2 cells. d Effect of LPS on protein expression of FOXM1, MATα2, MAT2β, TNF-α, and IL-6 with or without siRNA knockdown of FOXM1, MAT2A or MAT2B in RAW 264.7 cells for 24 h. e Effects of LPS on protein expression of FOXM1, MATα2, MAT2β, TNF-α, and IL-6 with FOXM1 overexpression and MAT2A or MAT2B siRNA treatment for 24 h in RAW 264.7 cells (left panel) and KCs isolated from Flox control mice (right panel). Densitometry values for protein levels are summarized in Supplementary Fig. 12e, f and Supplementary Fig. 13a–d. n = 3 independent experiments. Source data are provided as a Source Data file.