Fig. 8: FOXM1, MATα2, MAT2β are secreted in EVs and exchanged between hepatocytes, HSCs, and KCs.
a Following BDL and culture of hepatocytes from flox mice and Foxm1Hep−/−, EVs were extracted from the medium and used to treat HSCs from Foxm1HSC−/−. b Following BDL and culture of HSCs from flox mice and Foxm1HSC−/−, EVs were extracted from the medium and used to treat hepatocytes from Foxm1Hep−/−. c, d Following BDL and culture of KCs from flox mice and Foxm1KC−/−, EVs were extracted from the medium and used to treat (c) HSCs from Foxm1HSC−/− or (d) hepatocytes from Foxm1Hep−/−. e EVs extracted from hepatocytes of Flox and Foxm1Hep−/− after BDL were used to treat KCs from Foxm1KC−/− and (f) EVs extracted from HSCs of Flox and Foxm1HSC−/− after BDL were used to treat KCs from Foxm1KC−/−. All EV treatments were for 24 h after which protein expression of FOXM1, MATα2 and MAT2β were measured in cell lysates by western blotting. Densitometry values for protein levels are summarized in Supplementary Fig. 14a–f, n = 3 independent experiments (g) Summary of key findings showing the FOXM1/MAT2A/MAT2B axis in the different liver cell types driving liver inflammation and fibrosis. Source data are provided as a Source Data file.
