Fig. 6: DGAT1 and DGAT2 play distinct roles in ISR-induced LD formation and cell survival. | Nature Communications

Fig. 6: DGAT1 and DGAT2 play distinct roles in ISR-induced LD formation and cell survival.

From: Specific activation of the integrated stress response uncovers regulation of central carbon metabolism and lipid droplet biogenesis

Fig. 6

a, b Bar plots representing the mean number of LD per cell per well in Dmr-PERK cells treated for 8 h with dimerizer (Dmr, 1 nM), DGAT1i (20 μM) or DGAT2i (20 μM) as indicated. Error bars show mean ± SD across three independent experiments. Statistical significance was evaluated by one-way ANOVA followed by Dunnett’s multiple comparison test. c Quantification of the percent confluence of Dmr-PERK cells treated with vehicle, dimerizer (Dmr, 0.024 nM), DGAT1i (20 μM) or DGAT2i (20 μM), as indicated. Error bars show mean ± SD of two replicates and is representative of three independent experiments. d Western blot for cleaved caspase-3 in lysates from Dmr-PERK cells treated with the indicated dose of dimerizer (Dmr), DGAT1i (20 μM) or DGAT2i (20 μM), as indicated. Quantification of the number of LD (e) and cell number (f) simultaneously imaged by high-content live-cell microscopy in Dmr-PERK cells treated with vehicle, dimerizer (Dmr), DGAT1i (20 μM) or DGAT2i (20 μM), as indicated. Error bars show mean ± SD of three independent experiments. g Western blot for ATF4 in lysates from Dmr-PERK cells treated for 24 h with dimerizer (Dmr, 0.05 nM), DGAT1i (20 μM) or DGAT2i (20 μM), as indicated. h Average z-core of ISR GeneCLIC genes calculated from nCounter gene expression profiling of Dmr-PERK cells treated for 24 h with dimerizer (Dmr, 0.05 nM), DGAT1i (20 μM) or DGAT2i (20 μM), as indicated. Error bars show mean ± SD of three technical replicates. Statistical significance was evaluated by one-way ANOVA followed by Dunnett’s multiple comparison test. Source data are provided as a Source Data file.

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