Fig. 1: Talin cryoEM structures.
a The talin polypeptide chain, spanning 2541 amino acids, comprises an amino-terminal head with a FERM domain consisting of the F0-F3 subdomains and a carboxy-terminal ‘rod’ domain containing helix bundles R1-R13 and the carboxy-terminal dimerization α-helix (DD). R8 is inserted between the second and third α-helices of the R7 four-helix bundle. The gray outline highlights the rigid core of talin (encompassing domains F3, R1-R11; labeled in blue font). The decimals (5.5, 3.7, 3.4, and 2.7) refer to the talin5.5, talin3.7, talin3.4, and talin2.7 structures with their ordered domains colored spectrally. Domain boundaries are indicated with residue numbers above or below the domain name. b–g Cartoon drawing of our (b) 2.7 Å, (c) 3.7 Å, (d) 3.4 Å, (e) 3.39 Å, and (f) 5.5 Å talin cryoEM structures, color-coded spectrally and oriented as (g) the previously reported 6.2 Å resolution structure (PDB entry 6r9t)11. h Orthogonal views of our talin5.5 structure.
