Table 1 Data collection and model refinement statistics for our murine talin structures
talin2.7 | talin3.4 | talin3.7 | talin5.5 | |
|---|---|---|---|---|
data collection | ||||
magnification | 60,000 | 60,000 | 60,000 | 60,000 |
total dose (e− per Å2) | 48 | 48 | 48 | 48 |
fractions/frames | 40 | 40 | 40 | 40 |
pixel size (Å per pixel) | 0.36 | 0.36 | 0.36 | 0.36 |
defocus range [μm] | −0.8 to −2.4 | −0.8 to −2.4 | −1.0 to −2.4 | −1.0 to −2.4 |
data processing | ||||
number of micrographs | 10,349 | 10,349 | 2867 | 2867 |
number of particles | 439,156 | 68,494 | 91,849 | 8,318 |
resolution | 2.7 Å | 3.4 Å | 3.7 Å | 5.5 Å |
FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
model refinement | ||||
polypeptide chains | 1 | 1 | 1 | 1 |
number of non-H atoms | 12,691 | 14,703 | 13,547 | 16,046 |
protein residues | 1740 | 2005 | 1852 | 2192 |
bonds (root mean square deviation) | ||||
length (# over 4σ) | 0.005 Å (0) | 0.005 Å (0) | 0.005 Å (0) | 0.004 Å (0) |
angles (# over 4σ) | 0.692° (2) | 0.702 ° (5) | 0. 725° (2) | 0. 845° (9) |
MolProbity score | 1.50 | 1.27 | 1.87 | 2.27 |
clash score | 9.44 | 12.11 | 15.43 | 32.14 |
Ramachandran plot | ||||
outliers | 0.00 | 0.00 | 0.00 | 0.00 |
allowed | 0.0092 | 0.0265 | 0.0303 | 0.0407 |
favored | 0.9908 | 0.9735 | 0.9697 | 0.9593 |
rotamer outliers | 4 | 1 | 0 | 2 |
Cβ outliers | 0 | 0 | 0 | 0 |
CaBLAM outliers | 0.0133 | 0.018 | 0.0182 | 0.0142 |
EMDB identifier | EMD-43152 | EMD-43154 | EMD-43156 | EMD-43155 |
PDB identifier | 8vdo | 8vdp | 8vdr | 8vdq |
