Fig. 1: Map-based cloning of Pm6Sl.

a Schematic diagram of the wheat-Ae. longissima recombined chromosome 6Sl#3. b Resistance assay and marker analysis of 105 6Sl#3 recombinants narrowed Pm6Sl down to a 0.93 Mb interval. c Analysis of an additional five markers and evaluation of powdery mildew resistance in two additional distinct types (U and V) of 6Sl#3 recombinants delimited the Pm6Sl interval to 210 kb. The number of recombinants of each type is shown in brackets; all eight recombinants were resistant to the Bgt isolate E26. d Seven genes discovered within the 210 kb interval, of which only Ae.longissima.TL05.6S01G0714700 (named CNL1) and Ae.longissima.TL05.6S01G0715000 (named CNL2) are annotated to encode resistance proteins.