Fig. 6: Investigation of the agronomic traits of T3012II-3 and development of the gene marker pm6sl-1. | Nature Communications

Fig. 6: Investigation of the agronomic traits of T3012II-3 and development of the gene marker pm6sl-1.

From: An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew

Fig. 6

a Visual phenotypes of adult plants, spikes and grains of T3012II-3 (Pm6Sl +ve) and CS (Pm6Sl −ve) plants. b Comparing the agronomic traits between T3012II-3 and CS in plant height (n = 10), number of tillers per plant (n = 10), spike length (n = 30), number of spikelets per spike (n = 30), number of grains per spike (n = 30), grain length (n = 30), grain width (n = 30), and 1000-grain weight (n = 30). The data are displayed as box and whisker plots with individual data points. The error bars represent the maximum and minimum values. Centerline, median; box limits, 25th and 75th percentiles. Significant differences were determined by two-tailed Student’s t test. ns: no significant difference. c Electrophoresis patterns of the Pm6Sl gene marker pm6sl-1. M: 2 kb DNA Ladder Marker; 1: CS; 2-4: Pm6Sl stocks TA7548, TA7548S, and T3012II-3; 5–24: common wheat varieties Aikang58, Guomai301, Ningchun4, Zhoumai22, Kenong9204, Nongda399, Jimai22, Fielder, Xiaoyan81, Xiaoyan60, Yannong19, Jing411, Xinong528, Fengchan3, Aiqianniu, Nonglin11, Pingan602, Pingan0518, Pastor and Japper. The PCR amplicons of Pm6Sl are indicated by red arrows. The experiment was independently repeated three times with similar results. Source data are provided as a Source Data file.

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