Fig. 1: Overall landscape of OGDHc.

a One slide of a representative tomogram from all the 137 tomograms. One intact OGDHc was marked by the orange box. The black bar represents 100 nm. b A representative OGDHc from 2014 complexes is reconstructed by back-projecting E2o (blue), E1o (orange), and E3 (green) to the tomogram according to their refined coordinates and Euler angles. The black bar represents 3 nm. c E2o density map from subtomogram averaging. Structures are shown as transparent surfaces fitted with the atomic model of single particle E2o (this article). d E1o density map from subtomogram averaging. Structures are shown as transparent surfaces fitted with atomic model E1o (PDB:7WGR). e E3 density map from subtomogram averaging. Structures are shown as transparent surfaces fitted with atomic model E3 (PDB:6I4Q). f Statistical numbers of E1o and E3 per complex. The mean values of E1o and E3 were 4.16 and 4.10 in a complex, respectively, and the whole ratio of E1o and E3 is nearly 1:1. All of the 2014 OGDHcs were analyzed, which contain 8379 E1os and 8252 E3s. A boxplot shows the outliers, minimum, first quartile, medium, third quartile, and maximum of the data. g Occupancy of the peripheral subunits. E2o in the complex is not fully occupied, and most of the complex occupancy is lower than 50%. The largest number of reconstructed complexes are those with about 30% occupancy. h Distance distribution of the peripheral subunit. E1 and E3 both have a peak at a distance from the center of the 24-mer cubic core and their mean values are not much different. E1 distribution is more concentrated, and E3 is more diffuse.